BiologicalActivity
Fluorescentdye;suppliedasanNHSesterforcouplingtoprimaryaminegroups.CompatIBLewithself-labelingtagsystems,e.g.HaloTag®andSNAP-tag®.Suitableforconfocalfluorescentimagingandsuperresolutionmicroscopy(SRM)techniques,suchasdSTORM(liveandfixedcells).Cellpermeable.Excitationmaximum=549nm;emissionmaximum=571nm;Quantumyield=0.88;Extinctioncoefficient=101,000M-1cm-1;A280correctionfactoris0.169.JaneliaFluor®646SE(Cat.No.6148)alsoavailable.
HaloTagisatrademarkofPromegaCorporation,andSNAP-tagisatrademarkofNewEnglandBiolabs,Inc.
LicensingInformation
SoldunderlicensefromtheHowardHughesMedicalInstitute,JaneliaResearchCampus
ApplicationofJaneliaFluor®DyesinCardiacTissue
ApplicationofJaneliaFluor®DyesinCardiacTissue-Leftimage-Widefieldfluorescenceimageofmuscletissuefromanadultpigheart,displayingthedistributionofcollagenVIintheinterstitialspacebetweenheartmusclecells.ThissamplewaslabelledwithaprimaryantibodyagainstcollagenVIandasecondaryantibodyconjugatedtoJF549(Cat.No.6147).Scalebar:50μm.Middleimage-Anadultpighearttissuesection,10μmthick,labelledwithantibodiesagainstSERCA2ATPase,exhibitstheintricatestructureofthesarcoplasmicreticulum.Asuper-resolutionimage(toppanel),obtainedbyexploitingspontaneousphoto-switchingofJF549(dSTORM),showsfarsuperiordetailthanthediffractionlimitedwidefieldimage(bottom).Scalebar:200nm.Rightimage-AsingleisolatedWistarratcardiomyocytestainedagainstα-actinin,displayingitsperiodicstructurelocalisedattheendsofsarcomeres(Z-discs).WidefieldfluorescenceofJF549(leftpanel),priortoilluminatingwitha561nmlasertoinducephotoswitchingandproduceadSTORMsuper-resolutionimage(right).Scalebar:1μm.AllimageskindlyprovidedbyProf.ChristianSoeller,UniversityofExeter,acquiredbyAlexClowsleyandAnnaMeletiou.">
ApplicationofJaneliaFluor®DyesinCardiacTissue-Leftimage-Widefieldfluorescenceimageofmuscletissuefromanadultpigheart,displayingthedistributionofcollagenVIintheinterstitialspacebetweenheartmusclecells.ThissamplewaslabelledwithaprimaryantibodyagainstcollagenVIandasecondaryantibodyconjugatedtoJF549(Cat.No.6147).Scalebar:50μm.Middleimage-Anadultpighearttissuesection,10μmthick,labelledwithantibodiesagainstSERCA2ATPase,exhibitstheintricatestructureofthesarcoplasmicreticulum.Asuper-resolutionimage(toppanel),obtainedbyexploitingspontaneousphoto-switchingofJF549(dSTORM),showsfarsuperiordetailthanthediffractionlimitedwidefieldimage(bottom).Scalebar:200nm.Rightimage-AsingleisolatedWistarratcardiomyocytestainedagainstα-actinin,displayingitsperiodicstructurelocalisedattheendsofsarcomeres(Z-discs).WidefieldfluorescenceofJF549(leftpanel),priortoIlluminatingwitha561nmlasertoinducephotoswitchingandproduceadSTORMsuper-resolutionimage(right).Scalebar:1μm.
AllimageskindlyprovidedbyProf.ChristianSoeller,UniversityofExeter;acquiredbyAlexClowsleyandAnnaMeletiou.
TechnicalData
| M.Wt | 551.56 |
| Formula | C31H25N3O7 |
| Storage | Storeat-20°C |
| CASNumber | 1811539-32-8 |
| PubChemID | 124201856 |
| InChIKey | DWUIXAGWHCMXHN-UHFFFAOYSA-N |
| Smiles | O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CCC4)C=C3O5)=C(C=C/6)C5=CC6=[N+]7CCC7)=C2 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
SolubilityData
| Solvent | MaxConc.mg/mL | MaxConc.mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 55.16 | 100 |
PreparingStockSolutions
Thefollowingdataisbasedontheproductmolecularweight551.56.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
| Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
|---|---|---|---|
| 1mM | 1.81mL | 9.07mL | 18.13mL |
| 5mM | 0.36mL | 1.81mL | 3.63mL |
| 10mM | 0.18mL | 0.91mL | 1.81mL |
| 50mM | 0.04mL | 0.18mL | 0.36mL |
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