Biological Activity
A neurosteroid anesthetic that directly activates and potentiates GABAA receptor-activated membrane current (IGABA). Efficacy but not potency is determined by the alpha subunit of the receptor (EC50 values are 1.4, 1.8, 2.1, 2.4 and 2.5 μM for α1β1γ3, α1β1γ1, β1γ1, α2β1γ2L and α1β1γ2L isoforms respectively).
Technical Data
| M. Wt | 332.48 |
| Formula | C21H32O3 |
| Storage | Store at RT |
| Purity | ≥98% (HPLC) |
| CAS Number | 23930-19-0 |
| PubChem ID | 104845 |
| InChI Key | DUHUCHOQIDJXAT-OLVMNOGESA-N |
| Smiles | O=[C@](C)[C@H]3CC[C@@]([C@@](C)3C4)([H])[C@]2([H])CC[C@@]1([H])C[C@H](O)CC[C@@](C)1[C@]([H])2C4=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 33.25 | 100 | |
| ethanol | 33.25 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 332.48. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.01 mL | 15.04 mL | 30.08 mL |
| 5 mM | 0.6 mL | 3.01 mL | 6.02 mL |
| 10 mM | 0.3 mL | 1.5 mL | 3.01 mL |
| 50 mM | 0.06 mL | 0.3 mL | 0.6 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Maitra et al (1999) Subunit dependent modulation of GABAA receptor function by neuroactive steroids. Brain Res. 819 75 PMID: 10082863
Wegner et al (2007) Auto-modulation of neuroactive steroids on GABAA receptors: a novel pharmacological effect. Neuropharmacology. 52 672 PMID: 17084864
Zecharia et al (2009) The involvement of hypothalamic sleep pathways in general anesthesia: Testing the hypothesis using the GABAA receptor β3N265M knock-in mouse. J.Neurosci. 29 2177 PMID: 19228970
Keywords: Alphaxalone, supplier, Direct, activators, potentiator, GABAA, Receptors, GABAA, Receptors, GABAA, Receptors, Tocris Bioscience
