Biological Activity
The standard selective 5-HT1A agonist. Also has moderate affinity for 5-HT7 receptors (pKi is 6.6 at the human 5-HT7 receptor expressed in HEK 293 cells). Reduces hippocampal 5-HT levels following systemic administration in rats in vivo. (R)-(+)-8-Hydroxy-DPAT hydrobromide (Cat. No. 1080) and 7-Hydroxy-DPAT hydrobromide (Cat. No. 0706) also available.
Technical Data
| M. Wt | 328.29 |
| Formula | C16H25NO.HBr |
| Storage | Desiccate at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 76135-31-4 |
| PubChem ID | 6917794 |
| InChI Key | BATPBOZTBNNDLN-UHFFFAOYSA-N |
| Smiles | Br.CCCN(CCC)C1CCC2=CC=CC(O)=C2C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| phosphate buffered saline | 4.92 | 15mM with gentle warming | |
| ethanol | 1.64 | 5 | |
| water | 6.57 | 20mM with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 328.29. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.05 mL | 15.23 mL | 30.46 mL |
| 5 mM | 0.61 mL | 3.05 mL | 6.09 mL |
| 10 mM | 0.3 mL | 1.52 mL | 3.05 mL |
| 50 mM | 0.06 mL | 0.3 mL | 0.61 mL |
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References
References are publications that support the products' biological activity.
Helton and Colbert (1994) Alteration of in-vitro 5-HT receptor pharmacology as a function of multiple treatment with 5-hydroxytryptamine of 8-hydroxy-2-(di-N-propylamino)tetralin in rat isolated aorta, uterus and fundus, and guinea pig isolated trachea. J.Pharm.Pharmacol. 46 902 PMID: 7897596
Middlemiss et al (1983) 8-OH-DPAT discriminates between subtypes of the 5HT1 recognition site. Eur.J.Pharmacol. 90 151 PMID: 6223827
Wood et al (2000) Antagonist activity of meta-chlorophenylpiperazine and partial agonist activity of 8-OH-DPAT at the 5-HT7 receptor. Eur.J.Pharmacol. 396 1 PMID: 10822046
Yoshitake and Kehr (2004) Differential effects of (R)-, (R, S)- and (S)-8-hydroxy-2-(di-n-propylamino)tetralin on hippocampal serotonin release and induction of hypothermia in awake rats. Life Sci. 74 2865 PMID: 15050424
Keywords: 8-Hydroxy-DPAT hydrobromide, supplier, moderate, affinity, 5-HT7, 5-HT1A, agonists, Selective, Serotonin, Receptors, 5-Hydroxytryptamine, 5-HT1, 8-OH-DPAT, hydrobromide, 5-HT1A, Receptors, Tocris Bioscience
