Biological Activity
Microtubule inhibitor; inhibits mitosis. Also inhibits autophagosome-lysosome fusion. Enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.
Compound Libraries
Nocodazole is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 301.32 |
| Formula | C14H11N3O3S |
| Storage | Store at RT |
| Purity | ≥95% (HPLC) |
| CAS Number | 31430-18-9 |
| PubChem ID | 4122 |
| InChI Key | KYRVNWMVYQXFEU-UHFFFAOYSA-N |
| Smiles | COC(=O)NC1=NC2=CC(=CC=C2N1)C(=O)C1=CC=CS1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 15.07 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 301.32. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.32 mL | 16.59 mL | 33.19 mL |
| 5 mM | 0.66 mL | 3.32 mL | 6.64 mL |
| 10 mM | 0.33 mL | 1.66 mL | 3.32 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.66 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Vasquez et al (1997) Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. Mol.Biol.Cell 8 973 PMID: 9201709
Webb et al (2004) Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. Int.J.Biochem.Cell Biol. 36 2541 PMID: 15325591
Mizushima et al (2010) Methods in mammalian autophagy research. Cell 140 313 PMID: 20144757
Lin et al (2014) Enhanced homology-directed human genome engineering by controlled timing of CRISPR/Cas9 delivery. Elife 3 e04766 PMID: 25497837
Keywords: Nocodazole, supplier, Microtubule, inhibitors, inhibits, Tau, Tubulin, Microtubules, Mitosis, crispr, cas9, homology-directed, repair, HDR, Microtubules, Tocris Bioscience
