BiologicalActivity
PotentandselectiveDNA-PKinhibitor(IC50=14nM).SelectiveforDNA-PKoverarangeofkinasesincludingmTOR,PI3-K,ATMandATR.Potentiatestheeffectsofdoxorubicin(Cat.No.2252)andetoposide(Cat.No.1226)invitroandetoposideinvivo.AlsoenhancesCRISPR-Cas9-mediatedhomology-directedrepair(HDR)efficiency2to3-fold,anddecreasesnonhomologousend-joining(NHEJ)frequency~40%.
TechnicalData
| M.Wt | 413.49 |
| Formula | C25H19NO3S |
| Storage | Storeat+4°C |
| Purity | ≥99%(HPLC) |
| CASNumber | 503468-95-9 |
| PubChemID | 11327430 |
| InChIKey | JAMULYFATHSZJM-UHFFFAOYSA-N |
| Smiles | O=C1C3=C(C(C4=CC=CC5=C4SC6=C5C=CC=C6)=CC=C3)OC(N2CCOCC2)=C1 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
SolubilityData
| Solvent | MaxConc.mg/mL | MaxConc.mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 2.07 | 5mMwithgentlewarming |
PreparingStockSolutions
Thefollowingdataisbasedontheproductmolecularweight413.49.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
| Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
|---|---|---|---|
| 1mM | 2.42mL | 12.09mL | 24.18mL |
| 5mM | 0.48mL | 2.42mL | 4.84mL |
| 10mM | 0.24mL | 1.21mL | 2.42mL |
| 50mM | 0.05mL | 0.24mL | 0.48mL |
MolarityCalculator
ReconstitutionCalculator
Thereconstitutioncalculatorallowsyoutoquicklycalculatethevolumeofareagenttoreconstituteyourvial.Simplyenterthemassofreagentandthetargetconcentrationandthecalculatorwilldeterminetherest.