BiologicalActivity
IrreversibleNek2inhibitor(IC50=770nM).InhibitsNek2viaalkylationofresidueCys22.Displays5-foldselectivityforNek2overCdk1.ExhibitsnosignificanteffectonAuroraBorPlk1kinases,bipolarspindleassemblyorspindleassemblycheckpoint.Cellpermeable.
TechnicalData
| M.Wt | 320.35 |
| Formula | C18H16N4O2 |
| Storage | Storeat-20°C |
| Purity | ≥98%(HPLC) |
| CASNumber | 1311143-71-1 |
| PubChemID | 53262909 |
| InChIKey | ORKOHXAJFGRZCL-LCYFTJDESA-N |
| Smiles | O=C2NC1=CC=C(NC(C#C)=O)C=C1/C2=C/C3=C(C)N=C(CC)N3 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
SolubilityData
| Solvent | MaxConc.mg/mL | MaxConc.mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.03 | 100 |
PreparingStockSolutions
Thefollowingdataisbasedontheproductmolecularweight320.35.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
| Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
|---|---|---|---|
| 1mM | 3.12mL | 15.61mL | 31.22mL |
| 5mM | 0.62mL | 3.12mL | 6.24mL |
| 10mM | 0.31mL | 1.56mL | 3.12mL |
| 50mM | 0.06mL | 0.31mL | 0.62mL |
MolarityCalculator
ReconstitutionCalculator
Thereconstitutioncalculatorallowsyoutoquicklycalculatethevolumeofareagenttoreconstituteyourvial.Simplyenterthemassofreagentandthetargetconcentrationandthecalculatorwilldeterminetherest.