BiologicalActivity
Photoactivatablefluorescentdye;suppliedasanNHSesterforcouplingtoprimaryaminegroups.Non-fluorescentuntilactivatedat365nm.CompatIBLewithself-labelingtagsystems(e.g.HaloTag®andSNAPtag®).Suitableforsinglemoleculetrackingandsuperresolutionmicroscopyinlivecells,specificallylivecellsptPALMandfixedcellPALM.CanbemultiplexedwithPAJaneliaFluor®549,SE(Cat.No.6149)toperformtwo-coloursptPALMinlivecellswithactivationat405nm.Cellpermeable.Excitationmaximum=649-651nm;emissionmaximum=663-665nm.Uponproteinconjugationtheefficienciesofphotoconversionimprovesubstantially.Lowbackgroundstaining.
HaloTagisatrademarkofPromegaCorporation,andSNAP-tagisatrademarkofNewEnglandBiolabs,Inc.
LicensingInformation
SoldunderlicensefromtheHowardHughesMedicalInstitute,JaneliaResearchCampus
TechnicalData
| M.Wt | 617.74 |
| Formula | C34H31N5O5Si |
| Storage | Storeat-20°C |
| PubChemID | 127021042 |
| InChIKey | XVIATAHKOZWKHH-UHFFFAOYSA-N |
| Smiles | O=C(ON1C(CCC1=O)=O)C2=CC=C(C(C3=[N+]=[N-])=O)C(C43C5=CC=C(N6CCC6)C=C5[Si](C)(C)C7=CC(N8CCC8)=CC=C47)=C2 |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
SolubilityData
| Solvent | MaxConc.mg/mL | MaxConc.mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 12.35 | 20 |
PreparingStockSolutions
Thefollowingdataisbasedontheproductmolecularweight617.74.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
| Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
|---|---|---|---|
| 1mM | 1.62mL | 8.09mL | 16.19mL |
| 5mM | 0.32mL | 1.62mL | 3.24mL |
| 10mM | 0.16mL | 0.81mL | 1.62mL |
| 50mM | 0.03mL | 0.16mL | 0.32mL |
MolarityCalculator
ReconstitutionCalculator
Thereconstitutioncalculatorallowsyoutoquicklycalculatethevolumeofareagenttoreconstituteyourvial.Simplyenterthemassofreagentandthetargetconcentrationandthecalculatorwilldeterminetherest.