Biological Activity
Selective GSK-3α/β inhibitor (IC50 values are 0.01, 2.4, 4.3 and 63 μM for GSK-3α/β, CDK5/p25, CDK2/cyclin A and CDK1/cyclin B respectively). Induces Wnt signaling and inhibits CD8+ T cell effector differentiation.
Compound Libraries
BIO-acetoxime is also offered as part of the Tocriscreen Stem Cell Toolbox. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 398.21 |
| Formula | C18H12BrN3O3 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 667463-85-6 |
| PubChem ID | 24906283 |
| InChI Key | ORWSYHWTBSHFIV-UHFFFAOYSA-N |
| Smiles | O=C(NC4=C3C=CC(Br)=C4)/C3=C2NC1=CC=CC=C1/C/2=NOC(C)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 7.96 | 20 |
Preparing Stock Solutions
The following data is based on the product molecular weight 398.21. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.51 mL | 12.56 mL | 25.11 mL |
| 5 mM | 0.5 mL | 2.51 mL | 5.02 mL |
| 10 mM | 0.25 mL | 1.26 mL | 2.51 mL |
| 50 mM | 0.05 mL | 0.25 mL | 0.5 mL |
Molarity Calculator
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Product Datasheets
References
References are publications that support the products' biological activity.
Meijer et al (2003) GSK-3-selective inhibitors derived from Tyrian purple indirubins. Chem.Biol. 10 1255 PMID: 14700633
Polychronopoulos et al (2004) Structural basis for the synthesis of indirubins as potent and selective inhibitors of glycogen synthase kinase-3 and cyclin-dependent kinases. J.Med.Chem. 47 935 PMID: 14761195
Gattinoni et al (2009) Wnt signaling arrests T cell differentiation and generates CD8+ memory stem cells. Nat.Med. 15 808 PMID: 19525962
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