Biological Activity
Protein kinase C (PKC) activator that binds with high affinity (Ki = 1.35 nM). Initially activates and subsequently induces downregulation of PKC isozymes. Sensitizes tumor cells to cytotoxic effects of anticancer agents. Restores hippocampal synapses and spatial learning and memory in an in vivo model of fragile X syndrome.
Technical Data
| M. Wt | 905.04 |
| Formula | C47H68O17 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 83314-01-6 |
| PubChem ID | 5280757 |
| InChI Key | MJQUEDHRCUIRLF-TVIXENOKSA-N |
| Smiles | O=C(C)O[C@H](C(C)2C)C[C@@H](C[C@@H](O)CC(O[C@@H]([C@H](O)C)C[C@@H](O4)C3)=O)O[C@@]2(O)C[C@H](C1)O[C@@H](/C=C/C(C)(C)[C@@]4(O)[C@@H](OC(/C=C/C=C/CCC)=O)/C3=C/C(OC)=O)C/C1=CC(OC)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble in DMSO and in ethanol |
Preparing Stock Solutions
The following data is based on the product molecular weight 905.04. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.1 mL | 5.52 mL | 11.05 mL |
| 5 mM | 0.22 mL | 1.1 mL | 2.21 mL |
| 10 mM | 0.11 mL | 0.55 mL | 1.1 mL |
| 50 mM | 0.02 mL | 0.11 mL | 0.22 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Lorenzo et al (1997) The catalytic domain of protein kinase Cδ confers protection from down-regulation induced by bryostatin 1. J.Biol.Chem. 272 33338 PMID: 9407126
Sun and Alkon (2006) Bryostatin-1: pharmacology and therapeutic potential as a CNS drug. CNS Drug Rev. 12 1 PMID: 16834754
Wender et al (1998) The design, computer modeling, solution structure, and biological evaluation of synthetic analogs of bryostatin 1. Proc.Natl.Acad.Sci.USA 95 6624 PMID: 9618462
Sun et al (2014) Bryostatin-1 restores hippocampal synapses and spatial learning and memory in adult fragile X mice. J Pharmacol Exp Ther. 349 393 PMID: 24659806
Keywords: Bryostatin 1, supplier, Protein, kinases, C, activators, PKC, Bryostatin1, NSC339555, NSC, 339555, Protein, Kinase, C, Protein, Kinase, C, Tocris Bioscience
