BiologicalActivity
HighaffinityfluorescentlytaggedaptamerforCD133(AC133epitope)(Kdvaluesare33.9and83.2nMinHep3BandHT29cellsrespectively).EfficientlyinternalizeduponbindingtoCD133.Exhibitssuperiorpenetrationoftumorspherescomparedwithanantibodytothesametarget.
Foroptimalbindingaptamersrequirerefoldingintotheirtertiarystructurepriortouse.Pleaserefertotheaccompanyingrefoldingprotocol.
LicensingInformation
Soldunderlicensefromthepatentholder,DeakinUniversity.
TechnicalData
| M.Wt | 5549.58 |
| Formula | C182H219F9N58O104P16 |
| Storage | Storeat-20°C |
| Aptamertype(DNA/RNA) | RNA |
| NumberofBases | 15 |
| SequenceModifications | 2-fluoropyrimidines,3'-inverteddeoxythymidinecap,5'-fluorescentDY647tag |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
Solubility
PleaserefertothesesolubilityguidelinesforCD133-A15,5'-DY647(Cat.No.6103).
PreparingStockSolutions
Thefollowingdataisbasedontheproductmolecularweight5549.58.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
| Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
|---|---|---|---|
| 1mM | 0.18mL | 0.9mL | 1.8mL |
| 5mM | 0.04mL | 0.18mL | 0.36mL |
| 10mM | 0.02mL | 0.09mL | 0.18mL |
| 50mM | 0mL | 0.02mL | 0.04mL |
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