BiologicalActivity
HighaffinityfluorescentlytaggedaptamerforCD133(extracellulardomaineepitope)(Kdvaluesare52.3and145.1nMinHep3BandHT29cells,respectively).EfficientlyinternalizeduponbindingtoCD133.Exhibitssuperiorpenetrationoftumorspherescomparedwithanantibodytothesametarget.
Foroptimalbindingaptamersrequirerefoldingintotheirtertiarystructurepriortouse.Pleaserefertotheaccompanyingrefoldingprotocol.
LicensingInformation
Soldunderlicensefromthepatentholder,DeakinUniversity.
TechnicalData
| M.Wt | 6847.32 |
| Formula | C221H263F10N73O131P20 |
| Storage | Storeat-20°C |
| Aptamertype(DNA/RNA) | RNA |
| NumberofBases | 19 |
| SequenceModifications | 2-fluoropyrimidines,3'-inverteddeoxythymidinecap,5'-fluorescentDY647tag |
Thetechnicaldataprovidedaboveisforguidanceonly.ForbatchspecificdatarefertotheCertificateofAnalysis.
AllTocrisproductsareintendedforlaboratoryresearchuseonly.
Solubility
PleaserefertothesesolubilityguidelinesforCD133-B19,5'-DY647(Cat.No.6104).
PreparingStockSolutions
Thefollowingdataisbasedontheproductmolecularweight6847.32.Batchspecificmolecularweightsmayvaryfrombatchtobatchduetosolventofhydration,whichwillaffectthesolventvolumesrequiredtopreparestocksolutions.
| Concentration/SolventVolume/Mass | 1mg | 5mg | 10mg |
|---|---|---|---|
| 1mM | 0.15mL | 0.73mL | 1.46mL |
| 5mM | 0.03mL | 0.15mL | 0.29mL |
| 10mM | 0.01mL | 0.07mL | 0.15mL |
| 50mM | 0mL | 0.01mL | 0.03mL |
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