Biological Activity
Major metabolite of nicotine. Shown to activate a subpopulation of α3/α6β2 nAChRs in monkey striatum. Displays cognition-enhancing effects in vivo; reduces amyloid β (Aβ) aggregation and improves memory in an Alzheimer's disease mouse model.
Technical Data
| M. Wt | 176.22 |
| Formula | C10H12N2O |
| Storage | Desiccate at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 486-56-6 |
| PubChem ID | 854019 |
| InChI Key | UIKROCXWUNQSPJ-VIFPVBQESA-N |
| Smiles | CN(C(CC2)=O)[C@@H]2[C@]1=CC=CN=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| 1eq. HCl | 8.81 | 50 | |
| DMSO | 17.62 | 100 | |
| ethanol | 4.41 | 25 |
Preparing Stock Solutions
The following data is based on the product molecular weight 176.22. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 5.67 mL | 28.37 mL | 56.75 mL |
| 5 mM | 1.13 mL | 5.67 mL | 11.35 mL |
| 10 mM | 0.57 mL | 2.84 mL | 5.67 mL |
| 50 mM | 0.11 mL | 0.57 mL | 1.13 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Buccafusco and Terry (2003) The potential role of cotinine in the cognitive and neuroprotective actions of nicotine. Life Sci. 72 2931 PMID: 12706481
Terry et al (2005) Cotinine, a neuroactive metabolite of nicotine: potential for treating disorders of impaired cognition. CNS Drug Rev. 11 229 PMID: 16389292
O'Leary et al (2008) Cotinine selectively activates a subpopulation of α3/α6β2 nicotinic receptors in monkey striatum. 325 646 PMID: 18305015
Echeverria et al (2011) Cotinine reduces amyloid-β aggregation and improves memory in Alzheimer's disease mice. J.Alzheimers Dis. 23 1 PMID: 20930295
Keywords: Cotinine, supplier, metabolite, nicotine, Acetylcholine, Nicotinic, Receptors, Non-Selective, Subtypes, nAChR, Nicotinic, Receptors, (Non-selective), Nicotinic, Receptors, (Non-selective), Tocris Bioscience
