Biological Activity
Selective inhibitor of Akt/PKB. Inhibits IGF-1-stimulated phosphorylation and activation of Akt (complete inhibition at 2.5 μM), suppressing downstream activation of mTOR, p70 S6 kinase and S6 ribosomal protein. Shows no activity at PDK1, SGK1 or PI 3-kinase. Inhibits cell growth (IC50 ~ 2-6 μM) and induces apoptosis in rhabdomyosarcoma cells.
Compound Libraries
10-DEBC hydrochloride is also offered as part of the Tocriscreen Plus and Tocriscreen Kinase Inhibitor Toolbox I. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 381.34 |
| Formula | C20H25N2OCl.HCl |
| Storage | Desiccate at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 925681-41-0 |
| PubChem ID | 16760284 |
| InChI Key | SVKSJUIYYCQZEC-UHFFFAOYSA-N |
| Smiles | Cl.CCN(CC)CCCCN1C2=CC=CC=C2OC2=C1C=C(Cl)C=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 38.13 | 100 | |
| water | 38.13 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 381.34. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.62 mL | 13.11 mL | 26.22 mL |
| 5 mM | 0.52 mL | 2.62 mL | 5.24 mL |
| 10 mM | 0.26 mL | 1.31 mL | 2.62 mL |
| 50 mM | 0.05 mL | 0.26 mL | 0.52 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Thimmaiah et al (1992) Synthesis and chemical characterization of N-substituted phenoxazines directed toward reversing vinca alkaloid resistance in multidrug-resistant cancer cells. J.Med.Chem. 35 3358 PMID: 1527786
Thimmaiah et al (2005) Identification of N10-substituted phenoxazines as potent and specific inhibitors of Akt signaling. J.Biol.Chem. 36 31924 PMID:
Keywords: 10-DEBC hydrochloride, supplier, Selective, Akt, PKB, inhibitors, inhibits, Protein, Kinase, B, Kinases, Akt, (Protein, Kinase, B), Protein, Kinase, B/Akt, Akt, (Protein, Kinase, B), Tocris Bioscience
