Biological Activity
Inhibitor of DNA-dependent protein kinase (DNA-PK) (IC50 = 15 μM), an enzyme involved in the non-homologous end-joining (NHEJ) pathway of double-stranded DNA break (DSB) repair in human cells. Displays 100-fold higher potency than its analog vanillin and does not affect PKC activity. Produces lethal effects on cisplatin-treated D5037 cells upon continuous exposure.
Technical Data
| M. Wt | 211.15 |
| Formula | C9H9NO5 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 20357-25-9 |
| PubChem ID | 88505 |
| InChI Key | YWSPWKXREVSQCA-UHFFFAOYSA-N |
| Smiles | COC1=C(OC)C=C(C(C=O)=C1)[N+]([O-])=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 21.11 | 100 | |
| ethanol | 5.28 | 25mM with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 211.15. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.74 mL | 23.68 mL | 47.36 mL |
| 5 mM | 0.95 mL | 4.74 mL | 9.47 mL |
| 10 mM | 0.47 mL | 2.37 mL | 4.74 mL |
| 50 mM | 0.09 mL | 0.47 mL | 0.95 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Durant and Karran (2003) Vanillins - a novel family of DNA-PK inhibitors. Nucleic Acids Res. 31 5501 PMID: 14500812
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