Biological Activity
Nitroindoline-caged GABA (Cat.No. 0344) with similar photochemical properties to MNI-glutamate (Cat. No. 1490); for example, the same quantum yield (0.085). Highly water soluble; exhibits fast photorelease that is efficient at near-UV and 405 nm wavelengths.
Licensing Information
Sold under licence from the Medical Research Council.
Technical Data
| M. Wt | 499.3 |
| Formula | C15H23N3O12P2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 927866-58-8 |
| PubChem ID | 15953814 |
| InChI Key | QLCHBTSDKGMLRN-UHFFFAOYSA-N |
| Smiles | O=C(CCCN)N2C1=C([N+]([O-])=O)C=CC(OC(COP(O)(O)=O)COP(O)(O)=O)=C1CC2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 1 | 2mM with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 499.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2 mL | 10.01 mL | 20.03 mL |
| 5 mM | 0.4 mL | 2 mL | 4.01 mL |
| 10 mM | 0.2 mL | 1 mL | 2 mL |
| 50 mM | 0.04 mL | 0.2 mL | 0.4 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Wieboldt et al (1994) Synthesis and photochemistry of photolabile derivatives of gamma-aminobutyric acid for chemical kinetic investigations of the gamma-aminobutyric acid receptor in the millisecond time region. Biochemistry 33 1526 PMID: 8312272
Trigo et al (2009) Laser photolysis of DPNI-GABA, a tool for investigating the properties and distribution of GABA receptors and for silencing neurons in situ. J.Neurosci.Meths. 181 159 PMID: 19422852
Trigo (2010) Presynaptic miniature GABAergic currents in developing interneurons. Neuron 66 235 PMID: 20435000
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