Biological Activity
Cytosine analog that once incorporated into DNA acts as a suicide substrate for DNA methyltransferase. Inhibits DNA methyltransferase and results in DNA hypomethylation and activation of silent genes. Chemotherapeutic agent; suppresses growth of human tumor cell lines. Demethylates differentiation-related genes; reverses embryonic stem cell differentiation.
Compound Libraries
Decitabine is also offered as part of the Tocriscreen Plus, Tocriscreen Epigenetics Toolbox and Tocriscreen Library of FDA-Approved Compounds. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 228.21 |
| Formula | C8H12N4O4 |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 2353-33-5 |
| PubChem ID | 451668 |
| InChI Key | XAUDJQYHKZQPEU-KVQBGUIXSA-N |
| Smiles | O[C@@H]1[C@@H](CO)O[C@@H](N2C=NC(N)=NC2=O)C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 11.41 | 50 | |
| ethanol | 1.14 | 5 | |
| water | 11.41 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 228.21. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.38 mL | 21.91 mL | 43.82 mL |
| 5 mM | 0.88 mL | 4.38 mL | 8.76 mL |
| 10 mM | 0.44 mL | 2.19 mL | 4.38 mL |
| 50 mM | 0.09 mL | 0.44 mL | 0.88 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Bender et al (1998) Inhibition of DNA methylation by 5-aza-2'-deoxycytidine supresses the growth of human tumour cell lines. Cancer Res. 58 95 PMID: 9426064
Momparler (2005) Pharmacology of 5-aza-2'-deoxycytidine (decitabine). Semin.Hematol. 42 S9 PMID: 16015507
Jackson-Grusby et al (1997) Mutagenicity of 5-aza-2'-deoxycytidine is mediated by the mammalian DNA methyltransferase. Proc.Natl.Acad.Sci.USA 94 4681 PMID:
Keywords: Decitabine, supplier, DNA, methyltransferase, inhibitors, inhibits, Transferases, stem, cells, NSC127716, epigenetics, 5-Aza-2'-deoxycytidine, 2'-Deoxy-5-azacytidine, chemotherapeutics, 2'-Deoxy-5-azacytidine, 5-Aza-2'-deoxycytidine, NSC, 127716, DNA, Methyltransferases, DNA, Methyltransferases, Tocris Bioscience
