Biological Activity
GTP cyclohydrolase I (GCH1) inhibitor. Prevents the de novo synthesis of tetrahydrobiopterin (BH4) and thus suppresses the activity of NO synthase. Exhibits more potent inhibition of GCH1 in the presence of GFRP (GTP cyclohydrolase feedback-regulatory protein).
Technical Data
| M. Wt | 126.12 |
| Formula | C4H6N4O |
| Storage | Desiccate at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 56-06-4 |
| PubChem ID | 2944 |
| InChI Key | SWELIMKTDYHAOY-UHFFFAOYSA-N |
| Smiles | NC1=NC(N)=NC(O)=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble in DMSO |
Preparing Stock Solutions
The following data is based on the product molecular weight 126.12. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 7.93 mL | 39.64 mL | 79.29 mL |
| 5 mM | 1.59 mL | 7.93 mL | 15.86 mL |
| 10 mM | 0.79 mL | 3.96 mL | 7.93 mL |
| 50 mM | 0.16 mL | 0.79 mL | 1.59 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Moore and Handy (1997) Selective inhibitors of neuronal nitric oxide synthase - is no NOS really good NOS for the nervous system? TiPs 18 204 PMID: 9226999
Werner-Felmayer et al (1993) Pteridine biosynthesis in human endothelial cells. Impact of NO-mediated formation of cyclic GMP. J.Biol.Chem. 268 1842 PMID: 7678411
Xie et al (1998) GTP cyclohydrolase I inhibition by the prototypic inhibitor 2,4-diamino-6-hydroxypyrimidine. J.Biol.Chem. 273 21091 PMID: 9694862
Keywords: 2,4-Diamino-6-hydroxypyrimidine, supplier, GTP, cyclohydrolase, I, inhibitor, GCH1, GTPCH, GCH, inhibitors, inhibits, biosynthesis, tetrahydrobiopterin, BH4, NOS, Nitric, Oxide, synthase, Signaling, Signalling, Other, Nitric, Oxide, Other, Hydrolases, Other, Nitric, Oxide, Tocris Bioscience
