Biological Activity
Inhibitor of cathepsins B and L; also thought to inhibit calpain. Inhibits lysosomal proteases and interferes with autolysosomal digestion when used in combination with pepstatin A (Cat. No. 1190). Lysosome and cell permeable.
Technical Data
| M. Wt | 342.43 |
| Formula | C17H30N2O5 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 88321-09-9 |
| PubChem ID | 65663 |
| InChI Key | SRVFFFJZQVENJC-IHRRRGAJSA-N |
| Smiles | O=[C@@](N[C@H]([C@](NCCC(C)C)=O)CC(C)C)[C@H]1O[C@@H]1C(OCC)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 6.85 | 20 |
Preparing Stock Solutions
The following data is based on the product molecular weight 342.43. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.92 mL | 14.6 mL | 29.2 mL |
| 5 mM | 0.58 mL | 2.92 mL | 5.84 mL |
| 10 mM | 0.29 mL | 1.46 mL | 2.92 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.58 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Kim et al (2008) Autophagy upregulation by inhibitors of caspase-3 and mTOR enhances radiotherapy in a mouse model of lung cancer. Autophagy 4 659 PMID: 18424912
Sato et al (2007) Autophagy is activated in colorectal cancer cells and contributes to the tolerance to nutrient deprivation. Cancer Res. 67 9677 PMID: 17942897
Tanada et al (2005) Lysosomal turnover, but not a cellular level, of endogenous LC3 is a marker for autophagy. Autophagy 1 84 PMID: 16874052
Wilcox and Mason (1992) Inhibition of cysteine proteinases in lysosomes and whole cells. Biochem.J. 285 495 PMID: 1637341
Keywords: E 64d, supplier, lysosomal, proteases, inhibitors, inhibits, cathepsins, autophagy, autolysosomal, digestion, E64d, Autophagy, Calcium-Sensitive, Proteases, Cathepsin, Cathepsin, Tocris Bioscience
