Biological Activity
Activator of epithelial KCa channels, stimulates a large and sustained trans-epithelial Cl- secretory response across T84 monolayers. Induces hyperpolarization to the same magnitude as ACh in aortic value endothelial cells. Promotes embryonic stem cell (ESC) differentiation into cardiomyocytes.
Compound Libraries
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Technical Data
| M. Wt | 162.19 |
| Formula | C9H10N2O |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 10045-45-1 |
| PubChem ID | 82320 |
| InChI Key | CXUCKELNYMZTRT-UHFFFAOYSA-N |
| Smiles | CCN1C(=O)NC2=C1C=CC=C2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| tris buffer solution | 5.35 | 33mM with gentle warming | |
| DMSO | 16.22 | 100 | |
| ethanol | 16.22 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 162.19. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 6.17 mL | 30.83 mL | 61.66 mL |
| 5 mM | 1.23 mL | 6.17 mL | 12.33 mL |
| 10 mM | 0.62 mL | 3.08 mL | 6.17 mL |
| 50 mM | 0.12 mL | 0.62 mL | 1.23 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Ayotunde and Adeagbo (1999) 1-Ethyl-2-benzimidazolinone stimulates endothelial KCa channels and nitric oxide formation in rat mesenteric vessels. Eur.J.Pharmacol. 379 151 PMID: 10497901
Devor et al (1996) Modulation of Cl- secretion by benzimidazolones. I. Direct activation of a Ca2+ - dependent K+ channel. Am.J.Physiol. 271 L775 PMID: 8944721
Kusama et al (2005) Reduced hyperpolarisation in endothelial cells of rabbit aortic value following chronic nitroglycerine administration. Br.J.Pharmacol. 146 487 PMID: 16056232
Kleger et al (2010) Modulation of calcium-activated potassium channels induces cardiogenesis of pluripotent stem cells and enrichment of pacemaker-like cells. Circulation. 122 1823 PMID: 20956206
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