Biological Activity
Endogenous chemotactic peptide and agonist for the formyl peptide receptor 1 (FPR1) (Ki = 38 nM). Stimulates aggregation of leukocytes.
Technical Data
| M. Wt | 437.55 |
| Formula | C21H31N3O5S |
| Sequence |
MLF (Modifications: Met-1 = N-formyl-Met) |
| Storage | Desiccate at -20°C |
| CAS Number | 59880-97-6 |
| PubChem ID | 443295 |
| InChI Key | PRQROPMIIGLWRP-BZSNNMDCSA-N |
| Smiles | CSCC[C@H](NC=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 4.38 mg/ml in DMSO |
Preparing Stock Solutions
The following data is based on the product molecular weight 437.55. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.29 mL | 11.43 mL | 22.85 mL |
| 5 mM | 0.46 mL | 2.29 mL | 4.57 mL |
| 10 mM | 0.23 mL | 1.14 mL | 2.29 mL |
| 50 mM | 0.05 mL | 0.23 mL | 0.46 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Kreutzer et al (1978) Quantitative comparisons of various biological responses of neutrophils to different active and inactive chemotactic factors. Immunopharmacology 1 39 PMID: 45788
Mills et al (2000) Characterization of the binding site on the formyl peptide receptor using three receptor mutants and analogs of Met-Leu-Phe and Met-Met-Trp-Leu-Leu. J.Biol.Chem. 275 39012 PMID: 10960471
Spilberg et al (1984) Receptor blockade as a mechanism of deactivation of human neutrophils by pepstatin and formyl-Met-Leu-Phe. Inflammation 8 73 PMID: 6325345
Keywords: N-Formyl-Met-Leu-Phe, supplier, Endogenous, FPR1, agonists, FPRL, Formyl, Peptide, Receptors, fMLP, fMLF, fMLP, Formyl, Peptide, Receptors, Formyl, Peptide, Receptors, Tocris Bioscience
