Biological Activity
Dynamin inhibitor (IC50 values are 0.38 and 2.6 μM for dynamin 1 and 2 respectively). Inhibits dynamin-mediated endocytosis in multiple cell types and reduces synaptic vesicle endocytosis in vitro. Prevents botulinum A internalization in hippocampal neurons. Also protects against botulinum A-induced muscle paralysis in rats. Analog of Dynasore (Cat. No. 2897).
Technical Data
| M. Wt | 338.31 |
| Formula | C18H14N2O5 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1256493-34-1 |
| PubChem ID | 72193863 |
| InChI Key | ATUDAZGVGGVSOP-XFXZXTDPSA-N |
| Smiles | OC1=CC2=CC=CC=C2C=C1C(N/N=C/C3=C(O)C=C(O)C(O)=C3)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.23 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 338.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.96 mL | 14.78 mL | 29.56 mL |
| 5 mM | 0.59 mL | 2.96 mL | 5.91 mL |
| 10 mM | 0.3 mL | 1.48 mL | 2.96 mL |
| 50 mM | 0.06 mL | 0.3 mL | 0.59 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Harper et al (2011) Dynamin inhibition blocks botulinum neurotoxin type A endocytosis in neurons and delays botulism. J.Biol.Chem. 286 35966 PMID: 21832053
McCluskey et al (2013) Building a better dynasore: the dyngo compounds potently inhibit dynamin and endocytosis. Traffic 14 1272 PMID: 24025110
Keywords: Hydroxy Dynasore, supplier, dynamin, gtpases, dynamin1, dynamin2, endocytosis, botulinum, GTPase, GTPase, Tocris Bioscience
