Biological Activity
Cooling agent that activates the novel cold receptors TRPM8 (CMR1) and TRPA1 (ANKTM1/TRPN1), members of the TRP ion channel family. Induces currents in CMR1-expressing HEK 293 cells (EC50 = 0.36 μM) more potently than menthol or low temperatures. Produces "wet shakes" in vivo.
Technical Data
| M. Wt | 311.3 |
| Formula | C16H13N3O4 |
| Storage | Store at RT |
| Purity | ≥98% (HPLC) |
| CAS Number | 36945-98-9 |
| PubChem ID | 161930 |
| InChI Key | RCEFMOGVOYEGJN-UHFFFAOYSA-N |
| Smiles | OC1=C(C=CC=C1)N1CC=C(NC1=O)C1=CC=CC(=C1)[N+]([O-])=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 31.13 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 311.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.21 mL | 16.06 mL | 32.12 mL |
| 5 mM | 0.64 mL | 3.21 mL | 6.42 mL |
| 10 mM | 0.32 mL | 1.61 mL | 3.21 mL |
| 50 mM | 0.06 mL | 0.32 mL | 0.64 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Behrendt et al (2004) Characterization of the mouse cold-menthol receptor TRPM8 and vanilloid receptor type-1 VR1 using a fluorometric imaging plate reader (FLIPR) assay. Br.J.Pharmacol. 141 737 PMID: 14757700
McKemy et al (2002) Identification of a cold receptor reveals a general role for TRP channels in thermosensation. Nature 416 52 PMID: 11882888
Story et al (2003) ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell 112 819 PMID: 12654248
Wei et al (1983) AG-3-5: a chemical producing sensations of cold. J.Pharm.Pharmacol. 35 110 PMID: 6131976
Keywords: Icilin, supplier, activators, novel, cold, receptor, Cooling, agent, TRP, Channels, Transient, Receptor, Potential, AG35, TRPA, TRPM, AG, 3-5, TRPA1, TRPM, TRPA1, Tocris Bioscience
