Biological Activity
Selective ATP-uncompetitive mitotic kinesin Eg5 inhibitor (IC50 = 1.3 μM). Induces mitotic arrest, caspase-3 activation and cell growth inhibition in HCT116 cells. Displays no effect on microtubule dynamics. Preferentially induces mitotic arrest in cancer cells.
Technical Data
| M. Wt | 277.34 |
| Formula | C13H15N3O2S |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 72926-24-0 |
| PubChem ID | 2930014 |
| InChI Key | JEFVYQYZCAVNTP-UHFFFAOYSA-N |
| Smiles | CC1(C2=CC=CC=C2)N(C(C)=O)N=C(NC(C)=O)S1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 27.73 | 100 | |
| ethanol | 2.77 | 10 |
Preparing Stock Solutions
The following data is based on the product molecular weight 277.34. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.61 mL | 18.03 mL | 36.06 mL |
| 5 mM | 0.72 mL | 3.61 mL | 7.21 mL |
| 10 mM | 0.36 mL | 1.8 mL | 3.61 mL |
| 50 mM | 0.07 mL | 0.36 mL | 0.72 mL |
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References
References are publications that support the products' biological activity.
Nakai et al (2009) K858, a novel inhibitor of mitotic kinesin Eg5 and antitumor agent, induces cell death in cancer cells. Cancer Res. 69 3901 PMID: 19351824
Keywords: K 858, supplier, mitotic, kinesin, Eg5, inhibitors, inhibits, cytoskeleton, motor, proteins, ATPase, K858, Kinesin, Kinesin, Tocris Bioscience
