Biological Activity
Potent, selective inhibitor of protein kinase A (Ki = 60 nM). Has no effect on PKG or PKC (Ki > 2 μM). Reversibly arrests human skin fibroblasts in the G1 phase.
Technical Data
| M. Wt | 537.61 |
| Formula | C32H31N3O5 |
| Storage | Desiccate at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 108068-98-0 |
| PubChem ID | 9850141 |
| InChI Key | ZHEHVZXPFVXKEY-MWOZDUBJSA-N |
| Smiles | [H][C@]18O[C@](N6C4=C3N1C2=CC=CC=C2C3=C7C(CNC7=O)=C4C5=CC=CC=C56)([C@]([C@@](OCCCCCC)=O)(O)C8)C |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 26.88 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 537.61. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.86 mL | 9.3 mL | 18.6 mL |
| 5 mM | 0.37 mL | 1.86 mL | 3.72 mL |
| 10 mM | 0.19 mL | 0.93 mL | 1.86 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.37 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Cabell et al (1993) Effects of selective inhibition of protein kinase C, cyclic AMP-dependent protein kinase, and Ca(2+)-calmodulin-dependent protein kinase on neurite development in cultured rat hippocampal neurons. Int.J.Dev. Neurosci. 11 357 PMID: 7689287
Gadbois et al (1992) Multiple kinase arrest points in the G1 phase of nontransformed mammalian cells are absent in transformed cells. Proc.Natl.Acad.Sci.U.S.A. 89 8626 PMID: 1528872
Kase et al (1987) K-252 compounds, novel and potent inhibitors of protein kinase C and cyclic nucleotide-dependent protein kinases. Biochem.Biophys.Res.Commun. 142 436 PMID: 3028414
Keywords: KT 5720, supplier, Selective, protein, kinases, A, inhibitors, inhibits, PKA, cAMP, KT5720, Protein, Kinase, A, Protein, Kinase, A, Tocris Bioscience
