Biological Activity
Potent endogenous ligand for the Kisspeptin receptor (KISS1, GPR54). Binds with high affinity to rat and human KISS1 receptors with Ki values of 1.59 and 2.33 nM respectively. Inhibits metastasis and invasion in mouse melanomas and stimulates gonadotropin secretion following i.c.v. administration.
Technical Data
| M. Wt | 1302.45 |
| Formula | C63H83N17O14 |
| Sequence |
YNWNSFGLRF (Modifications: Phe-10 = C-terminal amide) |
| Storage | Desiccate at -20°C |
| CAS Number | 374675-21-5 |
| PubChem ID | 25240297 |
| InChI Key | RITKWYDZSSQNJI-INXYWQKQSA-N |
| Smiles | [H]N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(N)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 1 mg/ml in water |
Preparing Stock Solutions
The following data is based on the product molecular weight 1302.45. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.77 mL | 3.84 mL | 7.68 mL |
| 5 mM | 0.15 mL | 0.77 mL | 1.54 mL |
| 10 mM | 0.08 mL | 0.38 mL | 0.77 mL |
| 50 mM | 0.02 mL | 0.08 mL | 0.15 mL |
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References
References are publications that support the products' biological activity.
Kotani et al (2001) The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. J.Biol.Chem. 276 34631 PMID: 11457843
Peilecka-Fortuna et al (2007) Kisspeptin acts directly and indirectly to increase GnRH neuron activity and its effects are modulated by estradiol. Endocrinology 149 1979 PMID: 18162521
Becker et al (2005) Activation of GPR54 promotes cell cycle arrest and apoptosis of human tumour cells through a specific transcriptional program not shared by other Gq-coupled receptors. Biochem.Biophys.Res.Comm. 326 677 PMID:
Keywords: Kisspeptin 10 (human), supplier, Endogenous, ligands, KISS1, receptors, hOT7T175/AXOR12/GPR54, Metastin, KISS-derived, Peptide, Kisspeptin10, Metastin, (45-54), Kp-10, Kisspeptin, Receptor, Kisspeptin, Receptor, Tocris Bioscience
