Biological Activity
Rapidly and efficiently releases glutamate (Cat. No. 0218) when photolysed (350 - 365 nm excitation). Water soluble and stable at neutral pH, and pharmacologically inactive at neuronal glutamate receptors (up to 200 μM). 5.5 times more photosensitive than MNI-caged L-glutamate (Cat. No. 1490). Laser activation of MDNI-glu evokes a rapid increase in intracellular Ca2+ concentration in astrocytes.
Technical Data
| M. Wt | 368.3 |
| Formula | C14H16N4O8 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 864085-92-7 |
| PubChem ID | 124221751 |
| InChI Key | SYNWQVVPBORTQE-QMMMGPOBSA-N |
| Smiles | O=C(CC[C@H](N)C(O)=O)N1C2=C([N+]([O-])=O)C=C([N+]([O-])=O)C(OC)=C2CC1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| water | 3.68 | 10mM with gentle warming | |
| DMSO | 18.41 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 368.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.72 mL | 13.58 mL | 27.15 mL |
| 5 mM | 0.54 mL | 2.72 mL | 5.43 mL |
| 10 mM | 0.27 mL | 1.36 mL | 2.72 mL |
| 50 mM | 0.05 mL | 0.27 mL | 0.54 mL |
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References
References are publications that support the products' biological activity.
Fedoryak et al (2005) Synthesis of a caged glutamate for efficient one- and two-photon photorelease on living cells. Chem. Commun. (Camb.) 7 3664 PMID: 16027904
Papageorgiou et al (2005) Synthetic and photochemical studies of substituted 1-acyl-7-nitroindolines. Photochem.Photobiol.Sci. 4 887 PMID: 16252044
Popovic et al (2015) Electrical behaviour of dendritic spines as revealed by voltage imaging. Nat.Commun. 6 8436 PMID: 26436431
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