Biological Activity
Selective blocker of inwardly rectifying Kir2 potassium channels (IC50 values are 1.8, 2.8, 2.9 and 4.0 μM at pH 7.4 for mKir2.1, hKir2.6, hKir2.2 and hKir2.3 respectively). Exhibits no effect on rKir1.1 (IC50 > 300 μM); displays weak activity at hKir7.1 and rKir4.1 (IC50 values are 33 and 76 μM).
Technical Data
| M. Wt | 313.82 |
| Formula | C19H19NO.HCl |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 1222781-70-5 |
| PubChem ID | 44247466 |
| InChI Key | NGQIBUUFXDPHKT-UHFFFAOYSA-N |
| Smiles | COC(C=C3)=CC=C3CNCC1=C(C=CC=C2)C2=CC=C1.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 31.38 | 100 | |
| ethanol | 6.28 | 20 |
Preparing Stock Solutions
The following data is based on the product molecular weight 313.82. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.19 mL | 15.93 mL | 31.87 mL |
| 5 mM | 0.64 mL | 3.19 mL | 6.37 mL |
| 10 mM | 0.32 mL | 1.59 mL | 3.19 mL |
| 50 mM | 0.06 mL | 0.32 mL | 0.64 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Wang et al (2011) Selective inhibition of the Kir2 family of inward rectifier potassium channels by a small molecule probe: the discovery, SAR, and pharmacological characterization of ML133. ACS Chem.Biol. 6 845 PMID: 21615117
Wu et al (2010) A potent and selective small molecule Kir2.1 inhibitor. Probe Reports from the NIH Molecular Libraries Pro PMID: 21433384
Keywords: ML 133 hydrochloride, supplier, ML133, hydrochloride, Kir2, Kir2.x, Kir2.1, Kir2.6, Kir2.2, Kir2.3, inhibitors, inhibits, inward, rectifier, potassium, channels, IRK1, KCNJ2, IRK2, hIRK, IRK3, hIRK2, Inward, rectifier, Potassium, Channels, Inward, rectifier, Potassium, Channels, Tocris Bioscience
