Biological Activity
Selective MLCK inhibitor (Ki = 0.3 μM). Exhibits more potent inhibition than ML 9 hydrochloride (Cat. No. 0431) Displays reversible, ATP-competitive inhibition of Ca2+-calmodulin-dependent and -independent smooth muscle MLCKs. Inhibits proplatelet formation and stabilization.
Technical Data
| M. Wt | 452.74 |
| Formula | C15H17IN2O2S.HCl |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 110448-33-4 |
| PubChem ID | 9803932 |
| InChI Key | KDDALCDYHZIZMH-UHFFFAOYSA-N |
| Smiles | IC1=C(C=CC=C2S(N3CCCNCC3)(=O)=O)C2=CC=C1.Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 22.64 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 452.74. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.21 mL | 11.04 mL | 22.09 mL |
| 5 mM | 0.44 mL | 2.21 mL | 4.42 mL |
| 10 mM | 0.22 mL | 1.1 mL | 2.21 mL |
| 50 mM | 0.04 mL | 0.22 mL | 0.44 mL |
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References
References are publications that support the products' biological activity.
Saitoh et al (1987) Selective inhibition of catalytic activity of smooth muscle myosin light chain kinase. J.Biol.Chem. 262 7796 PMID: 3108259
Meinders et al (2015) Sp1/Sp3 transcription factors regulate hallmarks of megakaryocyte maturation and platelet formation and function. Blood 125 1957 PMID: 25538045
Keywords: ML 7 hydrochloride, supplier, ML7, HCl, MLC, kinase, inhibitor, inhibits, myosin, light, chain, kinase, inhibitors, MLCKs, platelets, Myosin, Light, Chain, Kinase, Myosin, Light, Chain, Kinase, Tocris Bioscience
