Biological Activity
NMDA (Cat.No. 0114) caged with the photosensitive 4-methoxy-7-nitroindolinyl group. Stoichiometrically releases chirally pure NMDA. Suitable for uncaging with 300-380 nm excitation. Improved localization can be achieved by uncaging at 405 nm (with mM concentration of caged compound in the bath solution). Exhibits rapid uncaging rate relative to ionotropic glutamate receptor activation.
Licensing Information
Sold under license from the UK Medical Research Council
Technical Data
| M. Wt | 323.3 |
| Formula | C14H17N3O6 |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 1227675-52-6 |
| PubChem ID | 57369294 |
| InChI Key | MUCRWOILMOHLGD-SECBINFHSA-N |
| Smiles | O=C(C[C@H](C(O)=O)NC)N2C1=C([N+]([O-])=O)C=CC(OC)=C1CC2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.33 | 100 | |
| water | 1.62 | 5mM with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 323.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.09 mL | 15.47 mL | 30.93 mL |
| 5 mM | 0.62 mL | 3.09 mL | 6.19 mL |
| 10 mM | 0.31 mL | 1.55 mL | 3.09 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Palma-Cerda et al (2012) New caged neurotransmitter analogs selective for glutamate receptor sub-types based on methoxynitroindoline and nitrophenylethoxycarbonyl caging groups. Neuropharmacology. 63 624 PMID: 22609535
Keywords: MNI-caged-NMDA, supplier, Caged, NMDA, Compounds, Glutamate, Receptors, N-Methyl-D-Aspartate, iGluR, Ionotropic, MNI-NMDA, 4-Methoxy-7-nitroindolinyl-caged, N-methyl-D-aspartic, acid, NMDA, Receptors, Caged, Glutamate, Compounds, NMDA, Receptors, Tocris Bioscience
