Biological Activity
MNI-caged glutamate that rapidly and efficiently releases glutamate (Cat. No. 0218) when photolysed (300 - 380 nm excitation). Water-soluble, highly resistant to hydrolysis, stable at neutral pH, and pharmacologically inactive at neuronal glutamate receptors (up to mM concentrations). 2.5-fold more efficient at releasing L-glutamate than NI-caged L-glutamate.
View more information regarding MNI-caged-L-glutamate.
Licensing Information
Sold under license from the Medical Research Council
Technical Data
| M. Wt | 323.3 |
| Formula | C14H17N3O6 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 295325-62-1 |
| PubChem ID | 6604871 |
| InChI Key | GXIDBZKXGUNITQ-VIFPVBQESA-N |
| Smiles | O=C(CC[C@H](N)C(O)=O)N2C1=C([N+]([O-])=O)C=CC(OC)=C1CC2 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| phosphate buffered saline | 16.16 | 50 | |
| ethanol | 32.33 | 100 | |
| water | 16.16 | 50mM with gentle warming |
Preparing Stock Solutions
The following data is based on the product molecular weight 323.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.09 mL | 15.47 mL | 30.93 mL |
| 5 mM | 0.62 mL | 3.09 mL | 6.19 mL |
| 10 mM | 0.31 mL | 1.55 mL | 3.09 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
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References
References are publications that support the products' biological activity.
Canepari et al (2001) Photochemical and pharmacological evaluation of 7-nitroindolinyl- and 4-methoxy-7-nitroindolinyl-amino acids as novel, fast caged neurotransmitters. J.Neurosci.Methods 112 29 PMID: 11640955
Maier et al (2005) Comparative analysis of inhibitory effects of caged ligands for the NMDA receptor. J.Neurosci.Methods 142 1 PMID: 15652611
Matsuzaki et al (2001) Dendritic spine geometry is critical for AMPA receptor expression in hippocampal CA1 pyramidal neurons. Nat.Neurosci. 4 1086 PMID: 11687814
Papageorgiou and Corrie (2000) Effects of aromatic substitutions on the photocleavage of 1-acyl-7-nitroindolines. Tetrahedron 56 8197 PMID:
Palma-Cerda et al (2012) New caged neurotransmitter analogs selective for glutamate receptor sub-types based on methoxynitroindoline and nitrophenylethoxycarbonyl caging groups. Neuropharmacology. 63 624 PMID: 22609535
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