Biological Activity
Inhibitor of class III phosphatidylinositol 3-kinase (PI 3-kinase); also inhibits the autophagic sequestration of cell proteins in rat hepatocytes. Blocks apoptosis in cerebellar granule cells (CGCs) following serum and potassium deprivation.
Technical Data
| M. Wt | 149.15 |
| Formula | C6H7N5 |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 5142-23-4 |
| PubChem ID | 1673 |
| InChI Key | FSASIHFSFGAIJM-UHFFFAOYSA-N |
| Smiles | NC(N=CN2C)=C1C2=NC=N1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 2.98 | 20 |
Preparing Stock Solutions
The following data is based on the product molecular weight 149.15. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 6.7 mL | 33.52 mL | 67.05 mL |
| 5 mM | 1.34 mL | 6.7 mL | 13.41 mL |
| 10 mM | 0.67 mL | 3.35 mL | 6.7 mL |
| 50 mM | 0.13 mL | 0.67 mL | 1.34 mL |
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References
References are publications that support the products' biological activity.
Blommaart et al (1997) The phosphatidylinositol 3-kinase inhibitors wortmannin and LY294002 inhibit autophagy in isolated rat hepatocytes. Eur.J.Biochem. 243 240 PMID: 9030745
Canu et al (2005) Role of the autophagic-lysosomal system on low potassium-induced apoptosis in cultured cerebellar granule cells. J.Neurochem. 92 1228 PMID: 15715672
Seglen and Gordon (1982) 3-methyladenine: specific inhibitor of autophagic/lysosomal protein degradation in isolated rat hepatocytes. Proc.Natl.Acad.Sci. USA 79 1889 PMID:
Keywords: 3-Methyladenine, supplier, pi, 3-kinase, phosphatidylinositol, 3, kinase, inhibitors, inhibits, autophagy, 3-MA, pi3k, 3-MA, PI, 3-Kinase, Inositol, Lipids, Other, Apoptosis, Autophagy, PI, 3-Kinase, Tocris Bioscience
