Biological Activity
Mre11-Rad50-Nbs1 (MRN)-ATM pathway inhibitor that blocks the 3' and 5' exonuclease activity associated with Mre11. Prevents ATM activation in response to double strand breaks (IC50 = 12 μM) and induces G2 cell cycle arrest. Also blocks homology-directed repair in vitro.
Compound Libraries
Mirin is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 220.25 |
| Formula | C10H8N2O2S |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 1198097-97-0 |
| PubChem ID | 1206243 |
| InChI Key | YBHQCJILTOVLHD-YVMONPNESA-N |
| Smiles | OC(C=C1)=CC=C1/C=C2/C(NC(S2)=N)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 22.02 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 220.25. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.54 mL | 22.7 mL | 45.4 mL |
| 5 mM | 0.91 mL | 4.54 mL | 9.08 mL |
| 10 mM | 0.45 mL | 2.27 mL | 4.54 mL |
| 50 mM | 0.09 mL | 0.45 mL | 0.91 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Dupre et al (2008) A forward chemical genetic screen reveals an inhibitor of the Mre11-Rad50-Nbs1 complex. Nat.Chem.Biol. 4 119 PMID: 18176557
Stivers (2008) Small molecule versus DNA repair mechanisms. Nat.Chem.Biol. 4 86 PMID: 18202674
Garner et al (2009) Corrected structure of mirin, a small-molecule inhibitor of the Mre11-Rad50-Nbs1 complex. Nat.Chem.Biol. 5 129 PMID: 19219009
Keywords: Mirin, supplier, MRN-ATM, pathway, inhibitors, inhibits, exonuclease, activity, Mre11-Rad50-Nbs1, Esterases, ATM, activation, Checkpoint, Control, Kinases, ATM, &, ATR, Kinase, Exonucleases, ATM, &, ATR, Kinase, Tocris Bioscience
