Biological Activity
Antibiotic and antitumor agent. Covalently binds DNA forming intra- and interstrand crosslinks. Inhibits DNA synthesis. Also used for MEF/STO feeder layer preparation in stem cell culture.
Technical Data
| M. Wt | 334.33 |
| Formula | C15H18N4O5 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 50-07-7 |
| PubChem ID | 5746 |
| InChI Key | NWIBSHFKIJFRCO-WUDYKRTCSA-N |
| Smiles | O=C(C(N)=C1C)C2=C(N(C[C@H]4[C@@H]3N4)[C@@]3(OC)[C@@H]2COC(N)=O)C1=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 33.43 | 100 | |
| ethanol | 1.67 | 5 | |
| water | 1.67 | 5 |
Preparing Stock Solutions
The following data is based on the product molecular weight 334.33. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.99 mL | 14.96 mL | 29.91 mL |
| 5 mM | 0.6 mL | 2.99 mL | 5.98 mL |
| 10 mM | 0.3 mL | 1.5 mL | 2.99 mL |
| 50 mM | 0.06 mL | 0.3 mL | 0.6 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Tomasz et al (1987) Isolation and structure of a covalent cross-link adduct between mitomycin C and DNA. Science 235 1204 PMID: 3103215
Bizanek et al (1992) Isolation and structure of an intrastrand cross-link adduct of mitomycin C and DNA. Biochemistry 31 3084 PMID: 1554696
Bryja et al (2006) Derivation of mouse embryonic stem cells. Nat.Protoc. 1 2082 PMID: 17487198
Park et al (2016) Modeling and correction of structural variations in patient-derived iPSCs using CRISPR/Cas9. Nat.Protoc. 11 2154 PMID: 27711053
Keywords: Mitomycin C, supplier, inhibitors, inhibits, DNA, synthesis, cross-linking, antitumor, agents, Apoptosis, Inducer, MitomycinC, antibiotics, chemotherapeutics, stem, cells, feeder, layers, preparation, culture, Ametycine, Apoptosis, Inducers, DNA,, RNA, and, Protein, Synthesis, Antibiotics, Stem, Cell, Signaling, Apoptosis, Inducers, Tocris Bioscience
