Biological Activity
Potent, cell-permeable, small molecule mitosis inhibitor that does not interact with tubulin. Arrests cells in mitosis and specifically inhibits the motility of the mitotic kinesin Eg5, a motor protein required for mitotic spindle formation and maintenance (IC50 = 14 μM).
Licensing Information
Sold with the permission of Harvard University
Compound Libraries
Monastrol is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 292.35 |
| Formula | C14H16N2O3S |
| Storage | Store at RT |
| Purity | ≥98% (HPLC) |
| CAS Number | 254753-54-3 |
| PubChem ID | 5353454 |
| InChI Key | UAIYNMRLUHHRMF-AATRIKPKSA-N |
| Smiles | CCOC(=O)C1=C(C)NC(=S)NC1C1=CC(O)=CC=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 29.24 | 100 | |
| ethanol | 5.85 | 20 |
Preparing Stock Solutions
The following data is based on the product molecular weight 292.35. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.42 mL | 17.1 mL | 34.21 mL |
| 5 mM | 0.68 mL | 3.42 mL | 6.84 mL |
| 10 mM | 0.34 mL | 1.71 mL | 3.42 mL |
| 50 mM | 0.07 mL | 0.34 mL | 0.68 mL |
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References
References are publications that support the products' biological activity.
Kapoor et al (2000) Probing spindle assembly mechanisms with monastrol, a small molecule inhibitor of the mitotic kinesin, Eg5. J.Cell Biol. 150 975 PMID: 10973989
Mayer et al (1999) Small molecule inhibitor of mitotic spindle bipolarity identified in a phenotype-based screen. Science 286 971 PMID: 10542155
Keywords: Monastrol, supplier, Selective, inhibitors, inhibits, mitotic, kinesin, Eg5, Mitosis, Kinesin, Mitosis, Kinesin, Tocris Bioscience
