Biological Activity
LDHA inhibitor; reduces lactate production in HeLa cells. Inhibits cell growth of LPC006 and PANC-1 cells in vitro. Also enhances the antiproliferative effects of Gemcitabine (Cat. No. 3259) on LPC006 and PANC-1 cells.
Compound Libraries
NHI 2 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 335.28 |
| Formula | C17H12F3NO3 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 1269802-97-2 |
| PubChem ID | 51355147 |
| InChI Key | YPPFWRWCZNXINO-UHFFFAOYSA-N |
| Smiles | ON2C1=CC(C3=CC=CC=C3)=CC(C(F)(F)F)=C1C=C2C(OC)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 33.53 | 100 | |
| ethanol | 33.53 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 335.28. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.98 mL | 14.91 mL | 29.83 mL |
| 5 mM | 0.6 mL | 2.98 mL | 5.97 mL |
| 10 mM | 0.3 mL | 1.49 mL | 2.98 mL |
| 50 mM | 0.06 mL | 0.3 mL | 0.6 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Maftouh et al (2014) Synergistic interaction of novel lactate dehydrogenase inhibitors with gemcitabine against pancreatic cancer cells in hypoxia. Br.J.Cancer 110 172 PMID: 24178759
Calvaresi et al (2013) Dual targeting of the Warburg effect with a glucose-conjugated lactate dehydrogenase inhibitor. Chembiochem 14 2263 PMID: 24174263
Granchi et al (2013) Assessing the differential action on cancer cells of LDH-A inhibitors based on the N-hydroxyindole-2-carboxylate (NHI) and malonic (Mal) scaffolds. Org.Biomol.Chem. 11 6588 PMID: 23986182
Keywords: NHI 2, supplier, NHI2, C17H12F3NO3, LDHA, inhibitors, lactate, dehydrogenases, antiproliferative, inhibits, Lactate, Dehydrogenase, A, Lactate, Dehydrogenase, A, Tocris Bioscience
