Biological Activity
NPEC ((N)-1-(2-nitrophenyl)ethyl) caged version of dopamine (Cat. No 3548); releases dopamine leading to D1 receptor activation upon UV light illumination (360 nm). Induces PKA activation and c-Fos expression in cortical and striatal neurons, with striatal neurons demonstrating a significantly greater detection and sensitivity to sub-second dopamine signals as compared to cortical neurons.
Technical Data
| M. Wt | 346.33 |
| Formula | C17H18N2O6 |
| Storage | Store at -20°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 1257326-23-0 |
| PubChem ID | 90488901 |
| InChI Key | PDNNZLULXSQJGZ-UHFFFAOYSA-N |
| Smiles | CC(OC(NCCC2=CC=C(O)C(O)=C2)=O)C1=C([N+]([O-])=O)C=CC=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 34.63 | 100 | |
| ethanol | 34.63 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 346.33. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.89 mL | 14.44 mL | 28.87 mL |
| 5 mM | 0.58 mL | 2.89 mL | 5.77 mL |
| 10 mM | 0.29 mL | 1.44 mL | 2.89 mL |
| 50 mM | 0.06 mL | 0.29 mL | 0.58 mL |
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References
References are publications that support the products' biological activity.
Castro et al (2013) Striatal neurones have a specific ability to respond to phasic dopamine release. J.Physiol. 591 3197 PMID: 23551948
Keywords: NPEC-caged-dopamine, supplier, caged, dopamine, compounds, neurotransmitters, D, receptors, dopaminergic, Caged, Dopamine, Compounds, D1, and, D5, Receptors, Non-selective, Dopamine, Non-selective, Dopamine, Tocris Bioscience
