Biological Activity
Inhibits calcium-sensitive chloride currents (10 μM). Putative GPR35 agonist.
Compound Libraries
NPPB is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 300.31 |
| Formula | C16H16N2O4 |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 107254-86-4 |
| PubChem ID | 4549 |
| InChI Key | WBSMIPAMAXNXFS-UHFFFAOYSA-N |
| Smiles | OC(=O)C1=CC(=CC=C1NCCCC1=CC=CC=C1)[N+]([O-])=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 30.03 | 100 | |
| ethanol | 6.01 | 20 |
Preparing Stock Solutions
The following data is based on the product molecular weight 300.31. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.33 mL | 16.65 mL | 33.3 mL |
| 5 mM | 0.67 mL | 3.33 mL | 6.66 mL |
| 10 mM | 0.33 mL | 1.66 mL | 3.33 mL |
| 50 mM | 0.07 mL | 0.33 mL | 0.67 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Keeling et al (1991) Effects of NPPB (5-nitro-1-(3-phenylpropylamino)benzoic acid on chloride transport in intestinal tissues and the T84 cell line. Biochim.Biophys.Acta 115 42 PMID: 1720331
Kirkup et al (1996) Investigation of the effects of 5-nitro-2-(3-phenylpropylamino)-benzoic acid (NPPB) on membrane currents in rat portal vein. Br.J.Pharmacol. 117 175 PMID: 8825360
Taniguchi et al (2008) 5-nitro-2-(3-phenylpropylamino)benzoic acid is a GPR35 agonist Pharmacology 82 245 PMID: 18818509
Wangemann et al (1986) Cl--channel blockers in the thick ascending limb of the loop of Henle. Structure-activity relationship. Pflugers Arch. 407 S128 PMID: 2434915
Keywords: NPPB, supplier, Chloride, channel, blockers, Cl-, Channels, GPR35, Ca2+-Activated, Chloride, Channels, Ca2+-Activated, Chloride, Channels, Tocris Bioscience
