Biological Activity
Positive modulator of small- and intermediate- conductance Ca2+-activated K+ channels (KCa2 and KCa3.1 channels); increases Ca2+ sensitivity. Displays no activity at BK channels.
Compound Libraries
NS 309 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 231.04 |
| Formula | C8H4Cl2N2O2 |
| Storage | Store at +4°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 18711-16-5 |
| PubChem ID | 11637204 |
| InChI Key | CVOUSAVHMDXCKG-UHFFFAOYSA-N |
| Smiles | ClC1=C2C(C(C(N2)=O)=NO)=CC=C1Cl |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 23.1 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 231.04. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.33 mL | 21.64 mL | 43.28 mL |
| 5 mM | 0.87 mL | 4.33 mL | 8.66 mL |
| 10 mM | 0.43 mL | 2.16 mL | 4.33 mL |
| 50 mM | 0.09 mL | 0.43 mL | 0.87 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Grgic et al (2009) Endothelial Ca2+-activated K+ channels in normal and impaired EDHF-dilator responses - relevance to cardiovascular pathologies and drug discovery. Br.J.Pharmacol. 157 509 PMID: 19302590
Strobaek et al (2004) Activation of human IK and SK Ca2+-activated K+ channels by NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime). Biochim.Biophys.Acta 1665 1 PMID: 15471565
Morimura et al (2006) Voltage-dependent Ca2+-channel block by openers of intermediate and small conductance Ca2+-activated K+ channels in urinary bladder smooth muscle cells. J.Pharm.Sci. 100 237 PMID:
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