Biological Activity
Cyclin-dependent kinase 2 (cdk2) inhibitor; induces cell-cycle arrest at the G2-M phase. 10- to 36-fold more selective for cdk2-cyclin A over other cdks (IC50 = 0.41 μM for cdk2-cyclin A). Potentiates the apoptotic effect of taxol (Cat. No. 1097) in HeLa cells; down-regulates the antiapoptotic protein survivin.
Technical Data
| M. Wt | 422.52 |
| Formula | C23H30N6O2 |
| Storage | Desiccate at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 444723-13-1 |
| PubChem ID | 10202471 |
| InChI Key | XHEQSRJCJTWWAH-UHFFFAOYSA-N |
| Smiles | O=C(N(CC)CC)C(C=C4)=CC=C4NC1=NC(NC=N2)=C2C(OCC3CCCCC3)=N1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 42.25 | 100 | |
| ethanol | 42.25 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 422.52. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.37 mL | 11.83 mL | 23.67 mL |
| 5 mM | 0.47 mL | 2.37 mL | 4.73 mL |
| 10 mM | 0.24 mL | 1.18 mL | 2.37 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.47 mL |
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References
References are publications that support the products' biological activity.
Pennati et al (2005) Potentiation of paclitaxel-induced apoptosis by the novel cyclin-dependent kinase inhibitor NU6140: a possible role for survivin down-regulation. Mol.Cancer.Ther. 4 1328 PMID: 16170024
Keywords: NU 6140, supplier, NU6140, cdk2, inhibitor, cyclin-dependent, kinase, inhibitors, inhibits, anti-apoptotic, arrests, cell, cycle, Cyclin-dependent, Kinase, Cyclin-dependent, Kinase, Tocris Bioscience
