Biological Activity
Broad spectrum MMP inhibitor; Ki values are 0.45, 0.73, 1.1, 1.1, 2.1, 42 and 2500 nM for MMP-12, MMP-2, MMP-8, MMP-13, MMP-9, MMP-3 and MMP-7 respectively and IC50 = 1600 nM for MMP-1). Displays no activity at other proteases up to a concentration of 100 μM. Exhibits antiangiogenic and anti-invasive properties on lung metastasis of murine renal cell carcinoma.
Technical Data
| M. Wt | 416.47 |
| Formula | C22H28N2O6 |
| Storage | Store at RT |
| Purity | ≥99% (HPLC) |
| CAS Number | 223472-31-9 |
| PubChem ID | 9888141 |
| InChI Key | HDWWQELUBWGQGA-WMZOPIPTSA-N |
| Smiles | O=C(N[C@H](COCOCC)C[C@H](C)C(NO)=O)C1=CC=C(OC2=CC=CC=C2)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 41.65 | 100 | |
| ethanol | 20.82 | 50 |
Preparing Stock Solutions
The following data is based on the product molecular weight 416.47. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.4 mL | 12.01 mL | 24.01 mL |
| 5 mM | 0.48 mL | 2.4 mL | 4.8 mL |
| 10 mM | 0.24 mL | 1.2 mL | 2.4 mL |
| 50 mM | 0.05 mL | 0.24 mL | 0.48 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Yamada et al (2000) ONO-4817, an orally active matrix metalloproteinase inhibitor, prevents lipopolysaccharide-induced proteoglycan release from the joint cartilage in guinea pigs. Inflamm.Res. 49 144 PMID: 10858013
Muraishi et al (2001) Effect of a matrix metalloproteinase inhibitor (ONO-4817) on lung metastasis of murine renal cell carcinoma. Anticancer Res. 21 3845 PMID: 11911256
Medina and Radomski (2006) Role of matrix metalloproteinases in intestinal inflammation. J.Pharmacol.Exp.Ther. 318 933 PMID: 16644899
Keywords: ONO 4817, supplier, Broad, spectrum, MMP, inhibitors, inhibits, Matrixins, Matrix, Metalloproteinase, Metalloprotease, Proteinases, Proteases, ONO4817, Matrix, Metalloprotease, Matrix, Metalloprotease, Tocris Bioscience
