Biological Activity
Cyclin-dependent kinase inhibitor that competes for the ATP binding site of the kinase. Selectively inhibits cdc2/cyclin B (IC50 = 7 μM), cdk2/cyclin A (IC50 = 7 μM), cdk2/cyclin E (IC50 = 7 μM), cdk/p35 kinase (IC50 = 3 μM) and ERK1/MAP kinase (IC50 = 25 μM). Arrests human fibroblasts in the G1 phase.
Technical Data
| M. Wt | 298.35 |
| Formula | C15H18N6O |
| Storage | Desiccate at -20°C |
| CAS Number | 101622-51-9 |
| PubChem ID | 4592 |
| InChI Key | GTVPOLSIJWJJNY-UHFFFAOYSA-N |
| Smiles | CN1C=NC2=C(NCC3=CC=CC=C3)N=C(NCCO)N=C12 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 50 mM in ethanol |
Preparing Stock Solutions
The following data is based on the product molecular weight 298.35. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.35 mL | 16.76 mL | 33.52 mL |
| 5 mM | 0.67 mL | 3.35 mL | 6.7 mL |
| 10 mM | 0.34 mL | 1.68 mL | 3.35 mL |
| 50 mM | 0.07 mL | 0.34 mL | 0.67 mL |
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References
References are publications that support the products' biological activity.
Abraham et al (1995) Cellular effects of olomoucine, an inhibitor of cyclin-dependent kinases. Biol.Cell 83 105 PMID: 7549905
Alessi et al (1998) The cyclin-dependent kinase inhibitors olomoucine and roscovitine arrest human fibroblasts in G1 phase by specific inhibition of CDK2 kinase activity. Exp.Cell.Res. 245 8 PMID: 9828096
Vesely et al (1994) Inhibition of cyclin-dependent kinases by purine analogues. Eur.J.Biochem. 224 771 PMID: 7925396
Keywords: Olomoucine, supplier, Cyclin-dependent, protein, kinases, inhibitors, inhibits, Cdks, Cyclin-dependent, Kinase, Cyclin-dependent, Kinase, Tocris Bioscience
