Biological Activity
Potent inhibitor of cAMP-dependent protein kinase (PKA) (Ki = 2.5 nM); derived from the active portion of the heat-stable PKA inhibitor protein PKI.
Technical Data
| M. Wt | 1868.08 |
| Formula | C80H130N28O24 |
| Sequence |
TYADFIASGRTGRRNAI (Modifications: Ile-17 = C-terminal amide) |
| Storage | Desiccate at -20°C |
| CAS Number | 121932-06-7 |
| PubChem ID | 16155227 |
| InChI Key | VAKHFAFLRUNHLQ-PEBJKXEYSA-N |
| Smiles | [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(N)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 1 mg/ml in water |
Preparing Stock Solutions
The following data is based on the product molecular weight 1868.08. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.54 mL | 2.68 mL | 5.35 mL |
| 5 mM | 0.11 mL | 0.54 mL | 1.07 mL |
| 10 mM | 0.05 mL | 0.27 mL | 0.54 mL |
| 50 mM | 0.01 mL | 0.05 mL | 0.11 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Glass et al (1989) Primary structural determinants essential for potent inhibition of cAMP-dependent protein kinase by inhibitory peptides corresponding to the active portion of the heat-stable inhibitor protein. J.Biol.Chem. 264 8802 PMID: 2722799
Glass et al (1989) Protein kinase inhibitor-(6-22)-amide peptide analogs with standard and nonstandard amino acid substitutions for phenylalanine 10. J.Biol.Chem. 264 14579 PMID: 2760075
Otmakhova et al (2000) Inhibition of the cAMP pathway decreases early long-term potentiation at CA1 hippocampal synapses. J.Neurosci. 20 4446 PMID: 10844013
Keywords: PKA Inhibitor Fragment (6-22) amide, supplier, Potent, protein, kinases, A, inhibitors, inhibits, PKA, Protein, kinase, inhibitor-(6-22)-amide, PKI-(6-22)-amide, Protein, Kinase, A, Protein, Kinase, A, Tocris Bioscience
