Biological Activity
Pseudosubstrate peptide inhibitor of protein kinase C (IC50 = 0.18 μM). Attenuates vascular hyperproliferation and hypertrophy induced by high glucose. Inhibits DNA and protein synthesis in vascular smooth muscle cells (VSMCs) in a dose-dependent manner. Inactive control available ([Glu27]-PKC (19-36), Cat. No. 4059).
Technical Data
| M. Wt | 2151.48 |
| Formula | C93H159N35O24 |
| Sequence | RFARKGALRQKNVHEVKN |
| Storage | Store at -20°C |
| CAS Number | 113731-96-7 |
| PubChem ID | 71308428 |
| InChI Key | NHCJMYZDRROLEW-OFXZVSIYSA-N |
| Smiles | [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 2 mg/ml in water |
Preparing Stock Solutions
The following data is based on the product molecular weight 2151.48. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.46 mL | 2.32 mL | 4.65 mL |
| 5 mM | 0.09 mL | 0.46 mL | 0.93 mL |
| 10 mM | 0.05 mL | 0.23 mL | 0.46 mL |
| 50 mM | 0.01 mL | 0.05 mL | 0.09 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Yasunari et al (1996) Possible involvement of phospholipase D and protein kinase C in vascular growth induced by elevated glucose concentration Hypertension 28 159 PMID: 8707376
Keywords: PKC (19-36), supplier, PKC, inhibitors, inhibits, pseudosubstrate, protein, kinase, C, fragment, (19-36), PKCI, Protein, Kinase, C, Protein, Kinase, C, Tocris Bioscience
