Biological Activity
Inhibitor of protein kinase C (PKC) ζ; attached to cell permeabilisation Antennapedia domain vector peptide. Consists of amino acids 113 - 129 of PKC ζ pseudosubstrate domain linked by a disulphide bridge to the Antennapedia domain vector peptide. The Antennapedia peptide is actively taken up by intact cells, at 4 or 37°C, ensuring rapid and effective uptake of the inhibitor peptide. Once inside the cell, the disulphide bonds are subjected to reduction in the cytoplasm leading to release of the inhibitor peptide. Induces mast cell degranulation by a PKC ζ-independent pathway.
Technical Data
| M. Wt | 4673.59 |
| Formula | C208H336N74O44S3 |
| Sequence |
CRQIKIWFQNRRMKWKK CSIYRRGARRWRKLYRAN* (Modifications: Disulfide bridge between 1 -1*) |
| Storage | Store at -20°C |
| PubChem ID | 90488711 |
| InChI Key | BQLVLLVGFUIFFK-OXSGATHOSA-N |
| Smiles | [H]N[C@@H](CSSC[C@H](N[H])C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(O)=O |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 2 mg/ml in water |
Preparing Stock Solutions
The following data is based on the product molecular weight 4673.59. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.21 mL | 1.07 mL | 2.14 mL |
| 5 mM | 0.04 mL | 0.21 mL | 0.43 mL |
| 10 mM | 0.02 mL | 0.11 mL | 0.21 mL |
| 50 mM | 0 mL | 0.02 mL | 0.04 mL |
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References
References are publications that support the products' biological activity.
Laudanna et al (1998) Evidence of ζ protein kinase C involvement in polymorphonuclear neutrophil integrin-dependent adhesion and chemotaxis. J.Biol.Chem 273 30306 PMID: 9804792
Lim et al (2008) A myristoylated pseudosubstrate peptide of PKC-ζ induces degranulation in HMC-1 cells independently of PKC-ζ activity. Life Sci. 82 733 PMID: 18289606
Theodore et al (1995) Intraneuronal delivery of protein kinase C pseudosubstrate leads to growth cone collapse. J.Neurosci. 15 7158 PMID: 7472470
Keywords: PKC z pseudosubstrate, supplier, PKC, zeta, inhibitors, inhibits, Protein, Kinases, C, pseudosubstrate, PKCz, ProteinkinaseC, Protein, kinase, C, zeta, pseudosubstrate, Protein, Kinase, C, Protein, Kinase, C, Tocris Bioscience
