Biological Activity
Binds to β-catenin (KD = 450 nM). Inhibits the interaction between β-catenin and T cell factor 4 (Tcf4) and disrupts the Wnt signaling pathway.
Compound Libraries
PNU 74654 is also offered as part of the Tocriscreen Plus. Find out more about compound libraries available from Tocris.
Technical Data
| M. Wt | 320.34 |
| Formula | C19H16N2O3 |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 113906-27-7 |
| PubChem ID | 9836739 |
| InChI Key | JJEDWBQZCRESJL-DEDYPNTBSA-N |
| Smiles | O=C(N/N=C/C2=CC=C(C)O2)C1=C(OC3=CC=CC=C3)C=CC=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 32.03 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 320.34. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 3.12 mL | 15.61 mL | 31.22 mL |
| 5 mM | 0.62 mL | 3.12 mL | 6.24 mL |
| 10 mM | 0.31 mL | 1.56 mL | 3.12 mL |
| 50 mM | 0.06 mL | 0.31 mL | 0.62 mL |
Molarity Calculator
Reconstitution Calculator
Dilution Calculator
Product Datasheets
References
References are publications that support the products' biological activity.
Trosset et al (2006) Inhibition of protein-protein interactions: the discovery of druglike beta-catenin inhibitors by combining virtual and biophysical screening. Proteins 64 60 PMID: 16568448
Wells and McClendon (2007) Reaching for high-hanging fruit in drug discovery at protein-protein interfaces. Nature 450 1001 PMID: 18075579
Keywords: PNU 74654, supplier, PNU74654, beta-catenin, b-catenin, β-catenin, binders, wnt, signalling, signaling, Beta-catenin, Beta-catenin, Tocris Bioscience
