Biological Activity
A non-selective P2 purinergic antagonist. Blocks recombinant P2X1, P2X2, P2X3, P2X5 (IC50 = 1 - 2.6 μM), native P2Y2-like (IC50 ~ 0.9 mM), and recombinant P2Y4 (IC50 ~ 15 mM) receptors. Delays onset of calcium responses to mild hypoosmotic stress in cortical slices. iso-PPADS (Cat. No. 0683) also available.
Technical Data
| M. Wt | 599.3 |
| Formula | C14H10N3Na4O12PS2 |
| Storage | Desiccate at -20°C |
| CAS Number | 192575-19-2 |
| PubChem ID | 101086948 |
| InChI Key | KURWUCJJNVPCHT-UHFFFAOYSA-J |
| Smiles | [Na+].[Na+].[Na+].[Na+].CC1=C(O)C(C=O)=C(COP([O-])([O-])=O)C(N=NC2=C(C=C(C=C2)S([O-])(=O)=O)S([O-])(=O)=O)=N1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 100 mM in water |
Preparing Stock Solutions
The following data is based on the product molecular weight 599.3. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.67 mL | 8.34 mL | 16.69 mL |
| 5 mM | 0.33 mL | 1.67 mL | 3.34 mL |
| 10 mM | 0.17 mL | 0.83 mL | 1.67 mL |
| 50 mM | 0.03 mL | 0.17 mL | 0.33 mL |
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References
References are publications that support the products' biological activity.
Ziganshin et al (1994) Selective antagonism by PPADS at P2x-purinoceptors in rabbit isolated blood vessels. Br.J.Pharmacol. 111 923 PMID: 8019770
Lambrecht et al (1992) PPADS, a novel functionally selective antagonist of P2 purinoceptor-mediated responses. Eur.J.Pharmacol. 217 217 PMID: 1330591
McLaren et al (1994) Investigation of the actions of PPADS, a novel P2x-purinoceptor antagonist, in the guinea-pig isolated vas deferens. Br.J.Pharmacol. 111 913 PMID: 8019769
Ralevic and Burnstock (1998) Receptors for purines and pyrimidines. Pharmacol.Rev. 50 413 PMID: 9755289
Thrane et al (2011) Critical role of aquaporin-4 (AQP4) in astrocytic Ca2+ signaling events elicited by cerebral edema. Proc.Natl.Acad.Sci.U S A 108 846 PMID: 21187412
Keywords: PPADS tetrasodium salt, supplier, P2, purinergic, antagonists, P2X, Receptors, Purinergic, P2Y, purinoceptors, P2X, Receptors, Purinergic, P2Y, Receptors, P2X, Receptors, Tocris Bioscience
