Biological Activity
Broad spectrum, reversible DUB inhibitor. Exhibits limited activity against other proteases. Induces accumulation of polyubiquitinated proteins, but has no direct inhibitory effect on the proteasome. Cytotoxic in HEK293T and colorectal cancer cells. Stabilizes microtubule network in oligodendroglial cells.
Technical Data
| M. Wt | 223.28 |
| Formula | C7H5N5S2 |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 2645-32-1 |
| PubChem ID | 2817763 |
| InChI Key | ZXOBLNBVNROVLC-UHFFFAOYSA-N |
| Smiles | NC1=C(SC#N)C=C(SC#N)C(N)=N1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 2.23 | 10 |
Preparing Stock Solutions
The following data is based on the product molecular weight 223.28. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.48 mL | 22.39 mL | 44.79 mL |
| 5 mM | 0.9 mL | 4.48 mL | 8.96 mL |
| 10 mM | 0.45 mL | 2.24 mL | 4.48 mL |
| 50 mM | 0.09 mL | 0.45 mL | 0.9 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Altun et al (2011) Activity-based chemical proteomics accelerates inhibitor development for deubiquitylating enzymes. Chem.Biol. 18 1401 PMID: 22118674
Seiberlich et al (2012) The small molecule inhibitor PR-619 of deubiquitinating enzymes affects the microtubule network and causes protein aggregate formation in neural cells: implications for neurodegenerative diseases. Biochim.Biophys.Acta 1823 2057 PMID: 22565157
Keywords: PR 619, supplier, PR619, inhibitors, DUBs, deubiquitinating, enzymes, deubiquitination, proteasome, inhibits, broad, spectrum, proteases, Deubiquitinating, Enzymes, Deubiquitinating, Enzymes, Tocris Bioscience
