Biological Activity
AMP-activated protein kinase (AMPK) activator. Stimulates AMPK heterotrimer (α1β1γ1) activity (EC50 = 0.3 μM). Selectively increases the activity of γ1- but not γ3-containing complexes. Thought to directly activate AMPK by antagonizing autoinhibition.
Technical Data
| M. Wt | 497.91 |
| Formula | C23H16ClN3O6S |
| Storage | Store at -20°C |
| Purity | ≥98% (HPLC) |
| CAS Number | 331002-70-1 |
| PubChem ID | 5753734 |
| InChI Key | RTHRCOIONCZINZ-JMIUGGIZSA-N |
| Smiles | ClC1=C(C(O)=O)C=C(/N=C3/NC(/C(S3)=C/C2=CC=C(C4=C([N+]([O-])=O)C=C(C)C(C)=C4)O2)=O)C=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| 1.1eq. NaOH | 49.79 | 100 | |
| DMSO | 49.79 | 100 |
Preparing Stock Solutions
The following data is based on the product molecular weight 497.91. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 2.01 mL | 10.04 mL | 20.08 mL |
| 5 mM | 0.4 mL | 2.01 mL | 4.02 mL |
| 10 mM | 0.2 mL | 1 mL | 2.01 mL |
| 50 mM | 0.04 mL | 0.2 mL | 0.4 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Zorn and Wells (2010) Turning enzymes ON with small molecules. Nat.Chem.Biol. 6 179 PMID: 20154666
Pang et al (2007) Small molecule antagonizes autoinhibition and activates AMP-activated protein kinase in cells. J.Biol.Chem. 283 16051 PMID:
Jensen et al (2015) PT-1 selectively activates AMPK-γ1 complexes in mouse skeletal muscle, but activates all three γ subunit complexes in cultured human cells by inhibiting the respiratory chain. Biochem.J. 467 461 PMID: 25695398
Keywords: PT 1, supplier, AMPK, amp-activated, protein, kinase, activators, PT1, AMPK, Enzyme, Substrates, /, Activators, AMPK, Tocris Bioscience
