Biological Activity
Toll-like receptor 1/2 (TLR1/2) agonist; induces production of TNF-α and IL-6 in macrophages. Stimulates phosphorylation of p100/p110 and p60 in granulocytic-differentiated HL-60 cells. Promotes differentiation of naive CD4+ T cells into Th17 cells.
Technical Data
| M. Wt | 1510.24 |
| Formula | C81H156N10O13S |
| Sequence |
CSKKKK (Modifications: Cys = S-[2,3-Bis(palmitoyloxy)-(2-RS)-propyl]-N-palmitoyl]-Cys |
| Storage | Store at -20°C |
| CAS Number | 112208-00-1 |
| PubChem ID | 130704 |
| InChI Key | OEDPHAKKZGDBEV-GFPBKZJXSA-N |
| Smiles | [H]N([C@@H](CSCC(COC(=O)CCCCCCCCCCCCCCC)OC(=O)CCCCCCCCCCCCCCC)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(O)=O)C(=O)CCCCCCCCCCCCCCC |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solubility | Soluble to 1 mg/ml in 50% Ethanol / water |
Preparing Stock Solutions
The following data is based on the product molecular weight 1510.24. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 0.66 mL | 3.31 mL | 6.62 mL |
| 5 mM | 0.13 mL | 0.66 mL | 1.32 mL |
| 10 mM | 0.07 mL | 0.33 mL | 0.66 mL |
| 50 mM | 0.01 mL | 0.07 mL | 0.13 mL |
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References
References are publications that support the products' biological activity.
St Paul et al (2012) Toll-like receptor ligands induce the expression of interferon-gamma and interleukin-17 in chicken CD4+ T cells. BMC Res.Notes 1 616 PMID: 23116495
Offermanns et al (1992) Lipopeptides are effective stimulators of tyrosine phosphorylation in human myeloid cells. Biochem.J. 282 551 PMID: 1312332
Caproni et al (2012) MF59 and Pam3CSK4 boost adaptive responses to influenza subunit vaccine through an IFN type I-independent mechanism of action. J.Immunol. 188 3088 PMID: 22351935
Keywords: Pam3CSK4, supplier, toll, like, receptors, TLRs, agonists, ligands, induces, cytokines, production, IFNg, TNFa, TNFalpha, IL-6, interleukin, Toll-like, Receptors, Toll-like, Receptors, Tocris Bioscience
