Biological Activity
Reversible, non-ATP competitive inhibitor of mitotic kinesin-like protein 2 (MKLP-2) (Ki = 3.4 μM). Inhibits the basal ATPase activity of MKLP-2 (IC50 = 1.35 μM). Exhibits selectivity for MKLP-2 over 12 other members of the kinesin superfamily, including the closely-related MKLP-1. Cell permeable.
Technical Data
| M. Wt | 245.28 |
| Formula | C16H11N3 |
| Storage | Store at +4°C |
| Purity | ≥99% (HPLC) |
| CAS Number | 57046-73-8 |
| PubChem ID | 12248889 |
| InChI Key | YMYYQRZCCKBFBE-MDWZMJQESA-N |
| Smiles | N#C/C(C3=CNC2=CC=CC=C23)=CC1=CN=CC=C1 |
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
All Tocris products are intended for laboratory research use only.
Solubility Data
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 24.53 | 100 | |
| ethanol | 2.45 | 10 |
Preparing Stock Solutions
The following data is based on the product molecular weight 245.28. Batch specific molecular weights may vary from batch to batch due to solvent of hydration, which will affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 4.08 mL | 20.38 mL | 40.77 mL |
| 5 mM | 0.82 mL | 4.08 mL | 8.15 mL |
| 10 mM | 0.41 mL | 2.04 mL | 4.08 mL |
| 50 mM | 0.08 mL | 0.41 mL | 0.82 mL |
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Product Datasheets
References
References are publications that support the products' biological activity.
Tcherniuk et al (2010) Relocation of Aurora B and survivin from centromeres to the central spindle impaired by a kinesin-specific MKLP-2 inhibitor. Agnew Chem.Int.Ed.Engl. 49 8228 PMID: 20857469
Keywords: Paprotrain, supplier, inhibitors, inhibits, MKLP-2, mitotic, kinesin-like, protein, 2, reversible, selective, Kinesin, Kinesin, Tocris Bioscience
